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KMID : 0043320070300060733
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Archives of Pharmacal Research
2007 Volume.30 No. 6 p.733 ~ p.738
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Effect of Quercitrin Gallate on Zymosan A-Induced Peroxynitrite Production in Macrophages
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Kim Byung-Hak
Cho Sung-Min
Chang Yoon-Sook
Han Sang-Bae
Kim Young-Soo
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Abstract
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We previously isolated quercetin 3-O-β-(2”-galloyl)-rhamnopyranoside (QGR), a quercitrin gallate, from aerial parts of Persicaria lapathifolia (Polygonaceae) to prevent superoxide production in monocytes from venous blood of healthy human donors. In this study, effects of QGR and its building moieties (quercitrin, quercetin and gallic acid) on the production of peroxynitrite, a coupling oxidant between superoxide and nitric oxide (NO) radicals, were investigated in zymosan A-stimulated macrophages RAW 264.7. The QGR, quercitrin and quercetin inhibited peroxynitrite production in dose-dependent manners with IC50 values of 2.1 μM, 24.5 μM and 5.1 μM, respectively, but gallic acid even at 100 μM was inactive. QGR also inhibited both zymosan A- and phorbol 12-myristate 13-acetate-induced superoxide productions with IC50 values of 3.2 μM and 4.7 μM, respectively. However, QGR affected neither zymosan Ainduced NO production nor inducible NO synthase synthesis. Taken together, QGR could inhibit peroxynitrite production by blocking superoxide production without affecting NO production. Finally, this study could provide a pharmacological potential of QGR in the oxidative stress-implicated disorders.
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KEYWORD
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Quercetin 3-O-β-(2”-galloyl)-rhamnopyranoside, Peroxynitrite, Superoxide, Nitric oxide, Zymosan A, Macrophages RAW 264.7
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